細胞色素P450家族成員1A1(CYP1A1)重組蛋白
Recombinant Cytochrome P450 1A1 (CYP1A1)
CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
- 編號URPD295Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位線粒體, 內質網腔
- 預測分子量61.5kDa
- 實際分子量62kDa(差異分析請參閱說明書)
- 片段與標簽Ser251~His521 with N-terminal His and GST Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點6.8
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)多克隆抗體 | WB; IHC |
| UMAD295Ra21 | 細胞色素P450家族成員1A1(CYP1A1)單克隆抗體 | WB; IHC; ICC; IP. |
| USED295Ra | 細胞色素P450家族成員1A1(CYP1A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMD295Ra | 細胞色素P450家族成員1A1(CYP1A1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSD295Ra01 | 細胞色素P450家族成員1A1(CYP1A1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats [Pubmed:25733458] |
| The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 [PubMed: 25733458] |
| Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590] |
| International Journal of Chemical and Natural Science | Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone [portal:uploads] |
| International Journal of Pharma Sciences | Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues [portal:uploads] |
| Molecular Nutrition &?Food?Research | Phenethyl isothiocyanate?and?indole-3-carbinol?from?cruciferous?vegetables, but not furanocoumarins from apiaceous?vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590] |
| Biochemistry (Mosc) | Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells [Pubmed:29738693] |
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