27kDa熱休克蛋白(Hsp27)單克隆抗體
Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- 編號UMAA693Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA693Hu01-27kDa熱休克蛋白(Hsp27)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 324 ¥ 756 ¥ 1080 ¥ 2700 ¥ 10800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 30μg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Glioma Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30μg/ml Mouse Anti-Human Hsp27 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Human Lung lysate; Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Porcine Skeletal muscle lysate;
Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human HSPB1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human Hsp27 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog MAA693Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對Hsp27的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別Hsp27。
用法
Western blottin:g: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA693Hu61 | 27kDa熱休克蛋白(Hsp27)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA693Hu01 | 27kDa熱休克蛋白(Hsp27)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA693Hu61 | 27kDa熱休克蛋白(Hsp27)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA693Hu01 | 27kDa熱休克蛋白(Hsp27)多克隆抗體 | WB; IHC; ICC/IF |
| UFAA693Hu01 | 抗27kDa熱休克蛋白(Hsp27)多克隆抗體 | FCM |
| ULAA693Hu81 | 27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA693Hu71 | 27kDa熱休克蛋白(Hsp27)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UFAA693Hu81 | 抗27kDa熱休克蛋白(Hsp27)多克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| UMAA693Hu22 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; ICC/IF |
| UMAA693Hu21 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; IHC; FCM |
| UMAA693Hu23 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; IHC; FCM |
| UMAA693Hu24 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; IHC |
| UMAA693Hu25 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; IHC |
| UMAA693Hu27 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; ICC/IF |
| UMAA693Hu29 | 27kDa熱休克蛋白(Hsp27)單克隆抗體 | WB; ICC/IF |
| UFAA693Hu06 | 抗27kDa熱休克蛋白(Hsp27)單克隆抗體 | FCM |
| UFAA693Hu08 | 抗27kDa熱休克蛋白(Hsp27)單克隆抗體 | FCM |
| UFAA693Hu04 | 抗27kDa熱休克蛋白(Hsp27)單克隆抗體 | FCM |
| UFAA693Hu02 | 抗27kDa熱休克蛋白(Hsp27)單克隆抗體 | FCM |
| ULAA693Hu74 | 27kDa熱休克蛋白(Hsp27)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA693Hu | 27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA693Hu | 27kDa熱休克蛋白(Hsp27)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA693Hu | 27kDa熱休克蛋白(Hsp27)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA693Hu01 | 27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
| Nature?Communications | HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis [Pubmed:29650953] |
| Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
| Diabetes Research and Clinical Practice | The beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity [Pubmed: 31102684] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients [] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771] |
| Beilstein Archives | Immunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation [] |
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