脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Lipopolysaccharide (LPS) ,etc. by FLIA (Flow Luminescence Immunoassay)
LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號ULMB526Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.03-30ng/mL
- 靈敏度最小可檢測劑量小于等于0.01 ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-99 | 88 |
| EDTA plasma(n=5) | 96-103 | 101 |
| heparin plasma(n=5) | 91-99 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 78-96% | 87-103% | 88-99% |
| EDTA plasma(n=5) | 92-102% | 99-105% | 80-97% | 82-95% |
| heparin plasma(n=5) | 89-101% | 78-91% | 85-103% | 80-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的脂多糖(LPS)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
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| UCPB526Ge21 | 脂多糖(LPS)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAB526Ge02 | 脂多糖(LPS)多克隆抗體 | ELISA |
| UPAB526Ge01 | 脂多糖(LPS)多克隆抗體 | IHC |
| ULAB526Ge71 | 脂多糖(LPS)多克隆抗體(生物素標(biāo)記) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAB526Ge81 | 脂多糖(LPS)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAB526Ge26 | 脂多糖(LPS)單克隆抗體 | ELISA |
| UMAB526Ge21 | 脂多糖(LPS)單克隆抗體 | WB; IHC |
| UMAB526Ge23 | 脂多糖(LPS)單克隆抗體 | IHC |
| UMAB526Ge24 | 脂多糖(LPS)單克隆抗體 | ELISA;IHC |
| UMAB526Ge25 | 脂多糖(LPS)單克隆抗體 | IHC |
| UMAB526Ge22 | 脂多糖(LPS)單克隆抗體 | WB; IHC |
| UMAB526Ge27 | 脂多糖(LPS)單克隆抗體 | WB; IHC |
| ULAB526Ge72 | 脂多糖(LPS)單克隆抗體(生物素標(biāo)記) | IHC |
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| Pharmacol Res | Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability [34896249] |
| Nutrients | Psychological and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome Undergoing a Low-FODMAP Diet: The Role of the Intestinal Barrier [34371976] |
| Food Science & Technology | Lactobacillus casei relieves liver injury by regulating immunity and suppression of the enterogenic endotoxin‐induced inflammatory response in rats cotreated with?… [34646510] |
| Food & Function | ALLICIN, A DIETARY TRPA1 AGONIST, PREVENTS HIGH FAT DIET-INDUCED DYSREGULATION OF GUT HORMONES AND ASSOCIATED COMPLICATIONS [34705006] |
| Journal of Clinical and Translational Hepatology | Indole-3-propionic Acid-aggravated CCl [34966655] |
| Pathogens | The Outer Membrane Vesicles of Salmonella enterica Serovar Typhimurium Activate Chicken Immune Cells through Lipopolysaccharides and Membrane Proteins [Pubmed:35335663] |
| Small | Biomimic Trained Immunity‐MSCs Delivery Microcarriers for Acute Liver Failure Regeneration [Pubmed:35411651] |
| Ecotoxicol Environ Saf | Effects of the maternal gut microbiome and gut-placental axis on melatonin efficacy in alleviating cadmium-induced fetal growth restriction [Pubmed:35487173] |
| Microbiome | Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axis [Pubmed:35710492] |
| Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis [Pubmed:35355860] |
| Food & Function | Co-interventions with Clostridium butyrate and soluble dietary fiber targeting gut microbiota improve MAFLD via the Acly/Nrf2/NFκ B signaling pathway [Pubmed:35543143] |
| Biomolecules | Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study [] |
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