免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Immunoglobulin G (IgG) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA544Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.1-100ug/mL
- 靈敏度最小可檢測劑量小于等于0.033 ug/mL.
- 樣本類型serum, plasma and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-102 | 87 |
| EDTA plasma(n=5) | 80-99 | 92 |
| heparin plasma(n=5) | 82-94 | 89 |
| sodium citrate plasma(n=5) | 93-101 | 96 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 86-94% | 98-105% | 99-105% |
| EDTA plasma(n=5) | 96-103% | 87-96% | 96-103% | 80-88% |
| heparin plasma(n=5) | 91-98% | 89-101% | 80-89% | 80-92% |
| sodium citrate plasma(n=5) | 87-95% | 83-95% | 98-105% | 88-101% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UNPA544Hu01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USAA544Hu00 | 兔抗人IgG多克隆抗體(無標記) | WB |
| USAA544Hu20 | 小鼠抗人IgG多克隆抗體(無標記) | WB |
| UPAA544Hu01 | 免疫球蛋白G(IgG)多克隆抗體 | WB; IHC |
| USAA544Hu10 | 山羊抗人IgG多克隆抗體(無標記) | WB; IHC; ICC; IF. |
| USAA544Hu29 | 小鼠抗人IgG多克隆抗體(辣根過氧化物酶標記) | WB |
| USAA544Hu28 | 小鼠抗人IgG多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| USAA544Hu27 | 小鼠抗人IgG多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USAA544Hu06 | 兔抗人IgG多克隆抗體(吲哚菁綠標記) | WB; IHC; ICC; IF. |
| USAA544Hu09 | 兔抗人IgG多克隆抗體(辣根過氧化物酶標記) | WB |
| USAA544Hu26 | 小鼠抗人IgG多克隆抗體(吲哚菁綠標記) | WB; IHC; ICC; IF. |
| USAA544Hu05 | 兔抗人IgG多克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
| USAA544Hu04 | 兔抗人IgG多克隆抗體(藻紅素標記) | WB; IHC; ICC; IF. |
| USAA544Hu07 | 兔抗人IgG多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USAA544Hu08 | 兔抗人IgG多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA544Hu22 | 免疫球蛋白G(IgG)單克隆抗體 | WB |
| USEA544Hu | 免疫球蛋白G(IgG)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| UCEA544Hu | 免疫球蛋白G(IgG)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA544Hu | 免疫球蛋白G(IgG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA544Hu | 免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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