促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Gonadotropin Releasing Hormone (GnRH) ,etc. by FLIA (Flow Luminescence Immunoassay)
GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
（注：單次混測多因子不超過8個(gè)指標(biāo) ）
- 編號ULMA843Fi
- 物種Danio rerio (Zebrafish，斑馬魚) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長1.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-101 | 89 |
| EDTA plasma(n=5) | 79-105 | 82 |
| heparin plasma(n=5) | 91-105 | 101 |
| sodium citrate plasma(n=5) | 98-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-101% | 88-95% | 78-91% | 88-95% |
| EDTA plasma(n=5) | 92-99% | 95-104% | 86-102% | 86-104% |
| heparin plasma(n=5) | 80-103% | 80-94% | 96-103% | 83-104% |
| sodium citrate plasma(n=5) | 96-104% | 93-101% | 90-99% | 86-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈品
相關(guān)產(chǎn)品
| 編號 | 適用物種：Danio rerio (Zebrafish，斑馬魚) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA843Fi01 | 促性腺激素釋放激素(GnRH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA843Fi01 | 促性腺激素釋放激素(GnRH)多克隆抗體 | WB; IHC; ICC; IP. |
| UCEA843Fi | 促性腺激素釋放激素(GnRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA843Fi | 促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Environmental Research | Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141] |
| Toxicology Letters | Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392] |
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| GENES | Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadism [Pubmed: 32535046] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
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| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
