睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Testosterone (Testo)
T; Testesteron; Testeronun
- 編號UCEA458Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍370.4-30,000pg/mL
- 靈敏度最小可檢測劑量小于等于162.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 836 ¥ 1194 ¥ 5373 ¥ 10149 ¥ 83580
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測睪酮(Testo)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的睪酮(Testo)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 98-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的睪酮(Testo)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中睪酮(Testo)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-104% | 88-96% | 91-99% | 80-98% |
| EDTA plasma(n=5) | 87-98% | 99-105% | 92-101% | 90-99% |
| heparin plasma(n=5) | 80-91% | 91-99% | 81-91% | 90-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將睪酮抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原（標準品或樣本），待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA458Ge11 | 睪酮(Testo)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA458Ge21 | 睪酮(Testo)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA458Ge01 | 睪酮(Testo)多克隆抗體 | ELISA |
| UPAA458Ge02 | 睪酮(Testo)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA458Ge71 | 睪酮(Testo)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA458Ge27 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge22 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge23 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge24 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge25 | 睪酮(Testo)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA458Ge21 | 睪酮(Testo)單克隆抗體 | ELISA |
| UMEA458Ge | 睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA458Ge | 睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA458Ge | 睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA458Ge | 睪酮(Testo)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA458Ge | 睪酮(Testo)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA458Ge | 睪酮(Testo)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA458Ge11 | 睪酮(Testo)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UKSA458Ge12 | 睪酮(Testo)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| The Tohoku Journal of Experimental Medicine | Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats [Jstage: source] |
| Plos one | Insulin Receptor Substrate 2 Is Required for Testicular Development [PubMed: PMC3669358] |
| International Journal of Molecular and Cellular Medicine | Central effects of camphor on GnRH and sexual hormones in male rat [Pubmed: 24551777] |
| Fertility and Sterility | Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356] |
| Urologia internationalis | Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats [Pubmed: 24051982] |
| International Braz J Urol | The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. [Pubmed: 24642156] |
| Journal of Materials Chemistry B | Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering [Rsc: Source] |
| Biochemical and Biophysical Research Communications | Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis [ScienceDirect: S0006291X14001843] |
| PLoS One. | Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate [Pubmed:Pmc4100933] |
| Reproductive Toxicology | Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring [Pubmed:24126190] |
| Physiology & Behavior | The impact of maternal consumption of cafeteria diet on reproductive function in the offspring [Pubmed:24631302] |
| Int Braz J Urol. | The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats [Pubmed:24642156] |
| Environmental Research | Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141] |
| Acta Cir Bras. | Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. [Pubmed:25185055] |
| Genet Mol Biol. | Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility [Pubmed:25505846] |
| Turkish Journal of Urology | Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source] |
| Int J Clin Exp Med | Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831] |
| Int Braz J Urol | Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats [PubMed: 26200553] |
| Advances in Environmental Biology | Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract [Aensiweb: Aeb] |
| international journal of environmental research and public health | Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886] |
| Environ Toxicol | Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period [PubMed: 25782374] |
| J Endocrinol | Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction [PubMed: 25770118] |
| Acta Pharmaceutica Sinica B | Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh [science:S2211383516301812] |
| Plos One | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642] |
| Theriogenology | Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count [Pubmed:26948295] |
| PPAR Research | Testosterone?Replacement?Modulates?Cardiac?Metabolic?Remodeling?after?Myocardial?Infarction?byUpreCavia (Guinea pig )lating?PPARα [Pubmed:27413362] |
| TURKISH JOURNAL OF MEDICAL SCIENCES | THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS [fileID:693211] |
| Int J Mol Sci. | Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| Journal of Applied Life Sciences International | Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats [Akindele922016JALSI29782.pdf] |
| Integrative Zoology | Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. [pubmed:27611741] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061] |
| PPAR?Research | Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα [pubmed:27413362] |
| Andrologia | Effects?of?treatment?with?Hypoxis?hemerocallidea?extract?on?sexual?behaviour?and?reproductiveparameters?in?male?rats. [pubmed:28000943] |
| Turkish journal of medical sciences | The effect of kisspeptin on spermatogenesis and apoptosis in rats. [pubmed:28263511] |
| Kafkas Universitesi Veteriner Fakultesi Dergisi | The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. [13006045] |
| Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium [S2405580816300991] | |
| Reproductive Sciences | Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome [pubmed:28673204] |
| Biomedicine & Pharmacotherapy | Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats [pubmed:28938525] |
| Molecular and Cellular Endocrinology | Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction [pubmed:28606867] |
| Ankara üniv Vet Fak Derg | The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice [DOI: 10.1501/Vetfak_0000002791] |
| Acta histochemica | Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-) [pubmed:28506466] |
| DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Aquatic Toxicology | Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations [Pubmed:29775925] |
| Molecular?Reproduction?and?Development? | deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and?… [Pubmed:29411926] |
| Journal of?Ovarian?Research | DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035] |
| Medical Science Monitor | Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4)?… [Pubmed:29627845] |
| Oncology Letters | Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes?… [10.3892:ol.2018.9165] |
| Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135] |
| Asian?Journal of Andrology | Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates [Pubmed: 30198494] |
| 第三軍醫(yī)大學(xué)學(xué)報 | 基因敲除損傷小鼠睪丸睪酮產(chǎn)生與生精功能 [] |
| Stem?Cells?Translational?Medicine | Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats [Pubmed: 30569668] |
| Brazilian?Journal?of?Pharmaceutical?Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients [] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| Journal of?Cellular?Physiology | Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells [Pubmed: 30628054] |
| Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol?induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary [] |
| Evidence-Based Complementary and Alternative Medicine | iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine [] |
| International Journal of Reproductive Biomedicine | The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. [] |
| Kafkas Universitesi Veteriner Fakultesi Dergisi | The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice [] |
| Journal of Cellular and Molecular Medicine | Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein [Pubmed: 31373170] |
| Journal of neuroscience research | Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analyses [Pubmed: 31359506] |
| Andrologia | Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats [Pubmed: 31353623] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| science of the total environment | Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells [Pubmed: 32334352] |
| JOURNAL OF MOLECULAR NEUROSCIENCE | Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment [Pubmed: 33159671] |
| BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance?… [Pubmed: 33109277] |
| J Food Biochem | Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1)?… [Pubmed: 33089540] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) [Pubmed: 33011509] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
| Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic?… [Pubmed: 32698821] |
| Antioxidants (Basel) | Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356] |
| Andrologia | Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty [Pubmed: 32478927] |
| chemosphere | N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats [Pubmed: 32447106] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
| IVERMECTINA IMPEDE DANOS TESTICULARES EM RATOS [] | |
| The effects of gold nanoparticles on Leydig cells and male reproductive function in mice [] | |
| The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance [] | |
| JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-|êB pathways in aging mice induced by D-galactose [33508368] |
| Toxicol Appl Pharmacol | Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion [33965371] |
| African Journal of Traditional, Complementary and Alternative Medicines | A comparative study on the aphrodisiac activity of food plants< i> Mondia whitei, Chenopodium album, Ccucurbita pe... [] |
| Bioengineered | MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 [34637688] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
| Eur J Pharmacol | Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2 [34265299] |
| Theriogenology | Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with?… [Pubmed:35063921] |
| Reprod Sci | Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice [Pubmed:35257354] |
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