絲切蛋白1(CFL1)單克隆抗體
Monoclonal Antibody to Cofilin 1 (CFL1)
CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle
- 編號UMAB559Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C12
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB559Hu01-絲切蛋白1(CFL1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 317 ¥ 739 ¥ 1056 ¥ 2640 ¥ 10560
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue
Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20μg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Porcine Liver lysate Primary Ab: 2μg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Bovine Cerebrum lysate; Lane2: HepG2 cell lysate<br/>Primary Ab: 0.2μg/ml Mouse Anti-Human CFL1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: Porcine Skin lysate; Lane2: HepG2 cell lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CFL1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CFL1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB559Hu01 | 絲切蛋白1(CFL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB559Hu01 | 絲切蛋白1(CFL1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAB559Hu01 | 絲切蛋白1(CFL1)多克隆抗體 | WB; IHC |
| UFAB559Hu51 | 抗絲切蛋白1(CFL1)多克隆抗體(別藻藍蛋白標(biāo)記) | FCM |
| UMAB559Hu21 | 絲切蛋白1(CFL1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB559Hu22 | 絲切蛋白1(CFL1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB559Hu23 | 絲切蛋白1(CFL1)單克隆抗體 | WB; ICC/IF |
| UMAB559Hu27 | 絲切蛋白1(CFL1)單克隆抗體 | ICC/IF |
| UMAB559Hu24 | 絲切蛋白1(CFL1)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB559Hu25 | 絲切蛋白1(CFL1)單克隆抗體 | WB; IHC; ICC/IF |
| USEB559Hu | 絲切蛋白1(CFL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB559Hu | 絲切蛋白1(CFL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB559Hu | 絲切蛋白1(CFL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Brazilian Journal of?Medical?and Biological?Research | Detection of sputum cofilin-1 as indicator of malignancy [Pubmed:29846436] |
| Nutrients | Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia [Pubmed: 32784915] |
| The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer [] |
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