T-細(xì)胞表面糖蛋白CD3ε(CD3e)活性蛋白
Active T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
T3E; TCRE; CD3-E Molecule,Epsilon(CD3-TCR Complex); T-cell surface antigen T3/Leu-4 epsilon chain
- 編號(hào)UAPD117Hu62
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)4.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 720 ¥ 1800 ¥ 3600 ¥ 10800 ¥ 27000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a 104-kD glycoprotein of the?immunoglobulin?(Ig)?superfamily?expressed in lymph node (RPKM 92.6), appendix (RPKM 45.9) and 9 other tissues. CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency). CD3E and CD247 can form two heterodimers flanking either side of the TCR. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human CD3e and recombinant human CD247. Briefly, CD3e was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD247-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD3e pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD3e and recombinant human CD247 was shown in Figure 1, the EC50?for this effect is 0.68 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPD117Hu61 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPD117Hu62 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPD117Hu61 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPD117Hu01 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPD117Hu62 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD117Hu01 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)多克隆抗體 | WB; IHC |
| UMAD117Hu22 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB; IHC |
| UMAD117Hu23 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB |
| URAD117Hu21 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)重組抗體 | ICC/IF; FCM |
| UMAD117Hu24 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB |
| UMAD117Hu25 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB |
| UMAD117Hu27 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB; ICC/IF |
| UMAD117Hu21 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | WB; IHC |
| URAD117Hu22 | T-細(xì)胞表面糖蛋白CD3ε(CD3e)重組抗體 | FCM |
| UFAD117Hu02 | 抗T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體 | FCM |
| UFAD117Hu86 | 抗T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| UFAD117Hu52 | 抗T-細(xì)胞表面糖蛋白CD3ε(CD3e)單克隆抗體(別藻藍(lán)蛋白標(biāo)記) | FCM |
