粘蛋白5AC(MUC5AC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 編號UKSA756Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.064ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3499 ¥ 5832 ¥ 10498 ¥ 20412 ¥ 34992
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對粘蛋白5AC(MUC5AC)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA756Mu02 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA756Mu01 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA756Mu03 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA756Mu01 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA756Mu02 | 粘蛋白5AC(MUC5AC)多克隆抗體 | IHC; ICC/IF |
| UPAA756Mu03 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA756Mu81 | 粘蛋白5AC(MUC5AC)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA756Mu71 | 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UIEA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA756Mu | 粘蛋白5AC(MUC5AC)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA756Mu | 粘蛋白5AC(MUC5AC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA756Mu01 | 粘蛋白5AC(MUC5AC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523] |
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| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234] |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Respiratory Research | IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells [pubmed:28931396] |
| Chemotherapy | Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. [pubmed:28578323] |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
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| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in?… [Pubmed:29349683] |
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