低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- 編號(hào)UKSA798Hu01
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.059ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)低氧誘導(dǎo)因子1α(HIF1a)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA798Hu02 | 低氧誘導(dǎo)因子1α(HIF1a)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA798Hu01 | 低氧誘導(dǎo)因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA798Hu02 | 低氧誘導(dǎo)因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA798Hu01 | 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體 | WB; IHC |
| UPAA798Hu02 | 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體 | WB; IHC |
| ULAA798Hu81 | 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA798Hu71 | 低氧誘導(dǎo)因子1α(HIF1a)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA798Hu21 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA798Hu23 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | WB; ICC/IF |
| UMAA798Hu24 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | WB |
| UMAA798Hu25 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | WB |
| UMAA798Hu22 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | WB; IHC |
| UMAA798Hu27 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | IHC; ICC/IF |
| UMAA798Hu29 | 低氧誘導(dǎo)因子1α(HIF1a)單克隆抗體 | IHC; ICC/IF |
| USEA798Hu | 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA798Hu | 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA798Hu | 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA798Hu | 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA798Hu | 低氧誘導(dǎo)因子1α(HIF1a)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA798Hu01 | 低氧誘導(dǎo)因子1α(HIF1a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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